Reagent Red Blood Cells for Irregular Antibody Screening

Intended Use:
It is intended for use in the detection of unexpected red blood cell blood group antibodies.
Summary of the Test:
Unexpected antibodies are found most frequently in samples from patients who were
exposed to foreign red blood cell antigens through transfusion or pregnancy
(approximately 1% of all patient samples). Less frequently red blood cell antibodies
are found in samples from blood donors.1-3 Some red blood cell antibodies are of
clinical importance since they may cause decreased red blood cell survival as the
result of hemolytic transfusion reactions, hemolytic disease of the newborn or
autoimmune hemolytic anemia. In vitro antibody detection (screening) tests are
employed to reveal the presence of these antibodies in patient or donor samples.4
Panoscreen is manufactured as a two-vial, three-vial or four-vial set of group O red
blood cells suitable for use in antibody detection procedures. The antigens for which
these donors have been typed are displayed on the Panoscreen Master List
accompanying each lot. Panoscreen I, II, III and Panoscreen 4 are manufactured
specifically for laboratories concerned with the optimal detection of antibodies
exhibiting dosage. (See Limitations) Panoscreen 4 is to be used as a four-vial set. It
includes the red blood cells of one Di(a+) donor.

Principle of the Test:
Serum or plasma is systematically tested against Panoscreen Reagent Red Blood
Cells. Agglutination of one or more of the Panoscreen cells at any phase, or
hemolysis at the saline or potentiated phases of testing, constitutes a positive test and
is the result of a reaction between an antigen and its respective antibody. No
agglutination or no hemolysis indicates either the absence of antibody, providing the
test red blood cells possess the corresponding antigen, or that an antibody, if present,
is in concentrations too low to be detected by the serologic techniques employed.
Once an antibody is detected, it can be identified by testing the sample with Panocell
Reagent Red Blood Cells.

Reagents:
I, II and III: a three-vial set.
Each vial contains a 2-4% suspension of single donor,
group O red blood cells prepared in a buffered preservative solution containing adenosine and adenine to retard hemolysis and/or loss of antigenicity during the dating period. The diluent does not interfere with complement-mediated hemolysis.
The donor red blood cells used in each reagent set are selected to contain most of the frequently inherited antigens. These red blood cells, when tested in parallel, will detect most of the commonly encountered antibodies other than anti-A or anti-B. The Panoscreen Master List indicates the donor code and antigenic composition for each red blood cell reagent.